Extended Data Fig. 4: The critical role of the insertion element in transcription activity of EBOV RNP.
(a) Clear green fluorescence can be visualized with the wild type L protein. However, for the insertion-element-deletion L(196-225)GS construct (residues 196 to 225 consisting of the insertion loop structure were deleted and two ends were linked with GS residues), no green fluorescence was observed. As a negative control, the L gene was not transfected in the replicon system. (b) The expression levels of NP, VP35 and VP30 were measured using Western Blotting assay and the tubulin was used as loading control. The data shown are representative of three independent experiments. (c) The transcription levels of L mRNA were analyzed by RT-PCR. The data represent mean values (histograms) ± s.d. (error bars) from three independent experiments.
