Extended Data Fig. 6: Gene expression for ionocyte markers in acorn worms (S. kowalevskii).
From: Ion regulation at gills precedes gas exchange and the origin of vertebrates
Anion exchanger (a; AE, P = 0.0163), sodium-proton exchanger (b; NHE, P < 0.0001), carbonic anhydrase (c; CA, P = 0.0431) and forkhead box protein I (d; FoxI, P < 0.0001) expression in skin, gill, intestine and proboscis of S. kowalevskii. Expression is relative to geometric mean of EF1A and 18S. Data presented as means±sd with individuals superimposed (n = 10). Letters indicate significant differences between tissues. One-way ANOVA with Tukey’s test (b,d) or Kruskal-Wallis with Dunn’s test (a,c), P < 0.05
