Extended Data Fig. 1: DdmDE defence system.
From: Plasmid targeting and destruction by the DdmDE bacterial defence system
a, Representation of VPI-2 defense island. Annotation was performed by PADLOC. b, Schematic representation of in vivo plasmid clearance assay. In brief, DdmD and/or DdmE were transformed into E. coli BL21 DE3 cells. c, representative dilution series of cultures either induced or uninduced. d, Quantification of transformation-fold reduction for DdmDE co-transformed, DdmD and DdmE alone, DdmDE co-transformed in the absence of antibiotic selection, and DdmD – DdmE∆INS domain. Significance between DdmDE and other variants was determined by ordinary one-way ANOVA test. Data are mean ± s.d. of at three independent experiments started from separate colonies. e, Denaturing urea-PAGE gel of DNA and RNA targets bound by DdmE (same samples as in Fig. 1a). No cleavage was observed. Representative of three independent experiments. For gel source data, see Supplementary Fig. 1.
