Extended Data Fig. 4: Binding of DNA by DdmD.
From: Plasmid targeting and destruction by the DdmDE bacterial defence system
a, Cryo-EM 2D class averages of DdmD in the absence and presence of DNA substrates with different overhang lengths. White arrow denotes monomeric DdmD. Structural changes between DdmD apo, short fork DNA and long for DNA. b, Conformational changes in DdmD dimer upon short fork DNA loading. c, Interactions between DdmD and DNA. Residues highlighted are tested in panel c. d, Plasmid interference assay to analyse DdmD-DdmE interactions. Significance between DdmDE and other variants was determined by ordinary one-way ANOVA test. ****P < 0.0001 Data are mean ± s.d. of at three independent experiments started from separate colonies. DdmDE, DdmD and DdmE are the same data as shown in Fig. 1h and Extended Data Fig. 1c,d.
