Extended Data Fig. 1: Definitions and nomenclatures used.
From: Mechanisms of actin filament severing and elongation by formins
(a) Domain diagrams of INF2 and Dia1, with zooms defining sub-domains within the FH2 domain. (b) The FH2 dimer forms a ring-like structure mediated by head-to-tail lasso-post interactions (left). FH2 subdomains colored as in part a (right). The newly-defined HCBH is highlighted in red. (c) Actin filament (PDB code: 8F8P) indicating short and long-pitch helices, pointed and barbed ends, plug, H-cleft, and D-loop. (d) Two perpendicular views of the actin monomer (PDB code: 1J6Z) with subdomains 1–4 labeled in blue circles. The H-cleft, nucleotide cleft, D-loop, and plug are indicated. (e) G- (blue, twisted) to F-actin (gray, flat) conformational transition, characterized by a 20° rotation of subdomains 1 and 2 relative to 3 and 4.
