Extended Data Fig. 6: Ketogenic diet activates P-eIF4E dependent translational regulation of ketogenic mRNAs.
From: Remodelling of the translatome controls diet and its impact on tumorigenesis
a. Quantification of indicated protein in the immunoblot of liver lysates from mice fed with regular chow or ketogenic diet (n = 3 mice). b. Quantification of indicated protein in the immunoblot of liver lysates from mice on ketogenic diet treated with vehicle or AMPK inhibitor (Comp. C) (n = 3 mice). c. Body weight of WT and eIF4ES209A mice fed with regular chow and ketogenic diet (n = 5 or 7 mice). d. Serum glycerol (n = 7 mice), e. Serum fatty acids (FA) (n = 7 mice) in WT and eIF4ES209A mice fed with ketogenic diet. f. Percentages of indicated mRNA distributed in sucrose gradient fractions of ketogenic diet fed WT and eIF4ES209A livers (n = 3 mice). g. Immunoblot of indicated proteins, h. Representative images of immunohistochemistry (IHC) of Hmgcs2, and i. Triglyceride levels (n = 3 or 4 mice) in the liver from eIF4ES209A mice injected with vehicle or Hmgcs2 cDNA with Myc tag. j. Immunoblot of indicated proteins and k. Representative images of IHC of Myc in the liver from eIF4ES209A mice injected with vehicle or PPARα cDNA with Myc tag. l. qPCR analysis of PPARα and Hmgcs2 mRNA levels in the liver from WT mice, and eIF4ES209A mice with hydrodynamic injection of vehicle or PPARα cDNA (n = 3 mice). All values represent the mean ± SEM; Two-way ANOVA for a, b, c, l, and two-sided Student’s t test for d, e, i.
