Extended Data Fig. 4: Single cell comparison of integration neurons across conditions.
From: Causal evidence of a line attractor encoding an affective state
a. Single cell contribution of x1 dimension (rSLDS weights) from engagement of aggression in example mouse. b. Single cell contribution of x1 dimension (rSLDS weights) from observation of aggression in example mouse. c. Overlap in neurons contributing to line attractor x1 & x2 dimension from rSLDS performing independently in engaging versus observing aggression. Left: Example mouse, Right: Average across 5 mice, error bars - sem. d. Dot product of x1 neural weight vectors during observation vs. engagement in aggression. rSLDS weights of the x1 dimension during observation were compared to model weights of the x1 and x2 dimensions during engagement using a dot product of the two weight vectors. (n = 5 mice, ***p = 0.0079, Two-tailed Mann-Whitney U-test, error bars - sem). e. Example raster of baseline activity from one mouse freely behaving while solitary in its home cage. f. Example single-cell traces from raster in Ex. Data Fig.e. Top - x1 neurons, bottom - x2 neurons. g. Comparison of frequency of Ca+2 transients (above 1.5σ in z-score activity) during baseline recordings across mice (mean frequency x1: 1.6 ± 0.2 events, mean frequency x2: 2.3 ± 0.2 events, n = 5 mice, *p = 0.012, Two-tailed Mann-Whitney U-test, error bars - sem). h. Comparison of the mean amplitude of Ca+2 transients in x1 vs. x2 neurons during baseline recordings, averaged across mice (mean amplitude x1: 0.58 ± 0.04 z-score, mean amplitude x2: 0.71 ± 0.08 z-score, n = 5 mice, p = 0.188, Two-tailed Mann-Whitney U-test, error bars - sem). i. Comparison of the decay time of Ca+2 events during baseline recordings across mice (mean tau x1: 1.7 ± 0.6s, mean tau x2: 2.3 ± 0.4s, n = 5 mice, p = 0.34, Two-tailed Mann-Whitney U-test, error bars - sem).
