Extended Data Fig. 1: LYCHOS purification and characterization.
From: LYCHOS is a human hybrid of a plant-like PIN transporter and a GPCR
a, Size-exclusion chromatography (Superose 6 10/300; Cytiva) of recombinant LYCHOS. Elution volume is approximately 14.3 ml. The peak fraction is indicated with grey shading. b, Gradient SDS-polyacrylamide gel electrophoresis of recombinant purified LYCHOS. Representative of >10 independent purifications. c, Trypsin digest and MS/MS of purified recombinant LYCHOS. Sequence coverage (~75%) of experimentally detected peptides is highlighted (coloured according to Fig. 1b) with some larger peptides being undetected. d, Mass photometry frequency distribution of wild-type LYCHOS. e, The 2.75 Å resolution reconstruction of wild-type LYCHOS. A single subunit is coloured from N- to C-terminus (yellow to purple), and the symmetrically related subunit is shown in white. f, Pipes and planks representation of e with numbered transmembrane helices.
