Extended Data Fig. 3: Cryo-EM volumes and validation.
From: LYCHOS is a human hybrid of a plant-like PIN transporter and a GPCR
a, Raw and denoised cryo-EM micrograph of vitrified LYCHOS. Scale bar 100 nm. Representative of >50,000 micrographs. b, Two-dimensional class averages calculated using prior assigned Euler angles without additional alignments. Scale bar 100 Å. c, Selected regions of cryo-EM map and model agreement showing side-chain density (grey) and corresponding model (blue: scaffold, magenta: transporter, orange: GPCR). d, Per-voxel local resolution variation. All volumes are coloured by the same uniformly sampled (0.5 Å increments) resolution range (2.5 to 4.5 Å). e, Map-to-model (solid green), unmasked (grey dashed line), and noise-substituted corrected (solid purple) gold-standard half-map Fourier shell correlation plots. f, Polar plots of normalized angular distribution frequency for the final refined particle sets.
