Extended Data Fig. 9: Additional analyses of GRAB_DA fluorescence during movement initiation in reserpine and L-DOPA treated mice.

a, Average GRAB_DA and tdTomato signals registered to the artificially shifted instantaneous velocity plotted in polar coordinates before and after i.p. injection of reserpine (3 mg/kg) and L-DOPA (250 mg/kg L-dopa methyl ester with 25 mg/kg carbidopa) for the experiment shown in Fig. 3b-j, 4 mice. b, Quantification of tdTomato fluorescence during contralateral movement initiations shown in Fig. 3g,h, event heatmaps are sorted by the order of the corresponding velocity signals in Fig. 3g, baseline 316 events from 4 mice, reserpine + L-DOPA 378/4. c–e, Individual (event heatmaps, top) and average (bottom) time courses of velocity amplitudes (c) and GRAB_DA (d) and tdTomato (e) fluorescence changes during ipsilateral movement initiations (right turns, velocity angles between 180° and 360°) for the experiment shown in Fig. 3b–j. Event heatmaps in c–e were sorted by the peak velocity amplitude in c, baseline 317/4, reserpine + L-DOPA 557/4. f–j, Individual (event heatmaps, top) and average (bottom) time courses of velocity amplitudes (f), and of GRAB_DA (g) and tdTomato (h) fluorescence during contralateral movement initiations (left turns, velocity angles between 0° and 180°), and peak velocity (i) and GRAB_DA (j) per mouse, for the experiment shown in Fig. 3k–n, RIM control data are replotted from Fig. 2j,l. Event heatmaps in f–h were sorted by the peak velocity amplitude in f, baseline 385/4, reserpine + L-DOPA 362/4. The observations that L-DOPA restored movement in RIM cKO^DA mice to pre-reserpine levels (f, Fig. 3k–n), and that dopamine denervation followed by apomorphine-induction of rotations was unaffected (Extended Data Fig. 1ac,ad), indicate that there is no strong sensitization of dopamine receptors or dopamine-modulated circuits after RIM ablation. Data are mean ± SEM; * p < 0.05, assessed by two-sided Mann-Whitney rank-sum tests in i, j.