Extended Data Fig. 2: Pharmacological inhibition of locomotion by drug infusion and assessment of excitation power output.
From: Dopamine dynamics are dispensable for movement but promote reward responses
a, Schematic of assessment of movement with bilateral drug infusion in dorsal striatum. b,c, Representative trajectories (b) and quantification of total distance traveled in 15 min (c) before and after local infusion of ACSF or D1 (SCH23390, 20 μM, 1 μl for each site) and D2 (haloperidol, 40 μM, 1 μl for each site) receptor antagonists, 4 mice. d,e, Example traces (d) and quantification (e) of fluorescence variation quantified as standard deviation (SD) of ΔF/F0 of GRABDA fluorescence at variable output power in freely moving mice, 7 mice. f, g, As in d,e, but for tdTomato, 7 mice; d–g reveal that ΔF/F0 variation is similar across excitation output powers. h, Schematic of the measurement of dopamine dynamics in freely moving mice. Fibre photometry and drug delivery were in the right dorsal striatum with an optofluid cannula. i–l, Example traces (i,j) and quantification of the variation of ΔF/F0 of GRABDA (k) and of tdTomato (l) fluorescence before and after local infusion of the sodium channel blocker TTX (500 nM, 1 μl), 6 mice. Data are mean ± SEM; * p < 0.05, ** p < 0.01, assessed by two-sided Mann-Whitney rank-sum tests for c, k; Kruskal-Wallis analysis of variance with post-hoc Dunn’s tests were used for e, g.
