Extended Data Fig. 3: Haloperidol injection and additional analyses of GRABDA dynamics in RIM cKODA mice.
From: Dopamine dynamics are dispensable for movement but promote reward responses
a–c, Example traces (a) and quantification of the variation of ΔF/F0 of GRABDA (b) and of tdTomato (c) fluorescence before and after i.p. injection of the D2 receptor antagonist haloperidol (2 mg/kg), RIM control 5 mice, RIM cKODA 5. d, Average GRABDA and tdTomato signals registered to the artificially shifted instantaneous velocity plotted in polar coordinates for the experiment shown in Fig. 2g,h. The shifting of the velocity time course to earlier or later time points relative to the photometry illustrates that the GRABDA fluorescence signal peaks after the velocity and suggests that dopamine signalling tracks the velocity time course, RIM control 5, RIM cKODA 5. e, Analyses of distance traveled for the experiment shown in Fig. 2, n as in a–c. f, Quantification of tdTomato fluorescence during contralateral movement initiations shown in Fig. 2i,j, event heatmaps are sorted by the order of the corresponding velocity signals in Fig. 2i, RIM control 354 events from 5 mice, RIM cKODA 455/5. g–i, Quantification of time courses of velocity amplitudes (g), and of GRABDA (h) and tdTomato (i) fluorescence changes during ipsilateral movement initiations (right turns, velocity angles between 180° and 360°). Event heatmaps were sorted by the peak velocity amplitude in g, RIM control 405/5, RIM cKODA 469/5. Data are mean ± SEM; ** p < 0.01, assessed by two-sided Mann-Whitney rank-sum tests for b, e.
