Extended Data Fig. 4: RAMP3 function as a neuropeptide receptor in T cells.
From: Neuropeptide signalling orchestrates T cell differentiation
(a) Expression levels of RAMP family in ILC2 cells using data set from GSE136154 (n = 4). (b) The expression of RAMP family genes in CD4+ T cells after 4 h in vitro activation with anti-CD3 and anti-CD28. Cells were collected for RNA extraction to assess the expression of receptors (Ramp1, Ramp2, Ramp3) with RT-qPCR (n = 6). (c) The expression levels of RAMP family in CD8+ T cells after 4 h in vitro activation with anti-CD3 and anti-CD28. Cells were collected for RNA extraction to assess the expression of receptors (Ramp1, Ramp2, Ramp3) with RT-qPCR (n = 6). (d) Expression dynamics of Ramp3 during T cell in vitro differentiation under Th0, Th1 and Th2 conditions. (e) Expression pattern of GPCRs during T helper cell differentiation. (f) Pearson correlation between the expression of Ramp3 and each GPCR gene during T cell in vitro differentiation. (g) Calcrl gene expression shows similar expression dynamics as Ramp3 during Th1 in vitro differentiation. (h) Ramp3 sgRNAs are further depleted in IFNγhigh cells and IL-13low/neg cells from both in vitro screens.
