Extended Data Fig. 8: PrdR over-expression changes the cell membrane lipid profile.

a. Protein abundance changes for the prdR deletant mutant, compared to the WT strain, demonstrating the specificity of the regulator for the prdAB membrane proteins (n = 5 independent biological replicates for proteomics). The x-axis is the log₂ fold-change and the y-axis is the -log₁₀ P value. b-d. The lipid species differentially produced by the WT, S491F mutant, WT EV, and WT^prdR shown as normalized abundance (intensity/total protein). Bars represent the median value and error bars represent the SEM. Each point represents independent biological replicates (n = 5). Data was analysed with a Two-way ANOVA ((WT versus RpoB mutant and WT EV versus WT^prdR) and P values were corrected for multiple testing using the Dunnett method. Exact P values are provided when the P value is above P < 0.0001; ns=not significant. The y-axis is the normalized intensity (Normalized Int.) for each lipid identified. e-f. Lipid species differentially produced by the ST203 and ST80 RpoB S491F clinical strain pairs, shown as normalized abundance (intensity/total protein). Bars represent the median value and error bars represent the SEM. Each point represents independent biological replicates (n = 5). Data was analysed with a Two-way ANOVA (DAP-S versus DAP-R) and P values were corrected for multiple testing using the Dunnett method. Lipid species with a significant difference are denoted. The y-axis is the normalized intensity (Normalized Int.) for each lipid identified.