Extended Data Fig. 2: DAP resistance RpoB substitutions are present globally.
From: Rifaximin prophylaxis causes resistance to the last-resort antibiotic daptomycin
a. Map of 4,476 VREfm genomes included. Circle size corresponds to total number of genomes and colour corresponds to region of isolation. Country coordinates are the country centroid position. This map is derived from the public domain project Natural Earth and available from www.naturalearthdata.com (“world”). b. The frequency of RpoB substitutions within the rifampicin resistance determining region (RRDR) in 4,476 VREfm genomes, sampled from 43 MLSTs. Bars are coloured by the number of isolates from each region of isolation containing the mutation. The identified daptomycin resistance associated mutations are coloured in red. c. The frequency of various MLSTs identified in the 630 VREfm carrying RpoB mutations in the rifampicin resistance determining region (RRDR). The identified daptomycin resistance mutations are coloured in red. d. Maximum-likelihood core-SNP-based phylogeny of clinical (n = 4,378) and animal VREfm (n = 98) inferred from an alignment of 8,435 SNPs, demonstrating the presence of RRDR RpoB mutations in clinical VREfm isolates. Overlaid is a heatmap showing the presence of at least one substitution in the RRDR of RpoB. VREfm that were animal-associated are highlighted in pink.
