Extended Data Fig. 2: Enrichment and correlation analysis of b-DNA LNP delivery in non-pregnant and pregnant mice.
From: Placenta-tropic VEGF mRNA lipid nanoparticles ameliorate murine pre-eclampsia
a–c, Volcano plots depicting significantly enriched (top right quadrant) and significantly depleted (top left quadrant) LNPs compared with the liver-tropic C12-200 LNP formulation in (a) non-pregnant and (b) pregnant tissues as well as (c) placentas and fetuses. Normalized delivery is reported as the mean (n = 6 biological replicates). For each tissue, two-sided, one-way ANOVAs with post hoc Student’s t tests using the Holm-Bonferroni correction for multiple comparisons with the C12-200 LNP were used to compare normalized delivery across LNP formulations for generating p values. d–g, The squared Pearson’s correlation coefficient for mean normalized delivery (r2) was calculated for each tissue pair and is presented as a heatmap for (d) non-pregnant mouse tissues, (e) pregnant mouse tissues, (f) between non-pregnant and pregnant mouse tissues, and (g) between pregnant mouse tissues and the placentas and fetuses. Dist., distal; Prox., proximal.
