Extended Data Fig. 1: Generation of all possible SNVs using CRISPR-Cas9 based SGE.
From: Saturation genome editing-based clinical classification of BRCA2 variants
a, Heatmap showing the total number of expected and recovered variants across 51 experimental pools to saturate the C-terminal DNA binding domain. The value in the boxes represents the number of variants expected/recovered ranging from 80 SNVs (light blue) to 234 SNVs (dark blue). A total of 96.4% of all possible SNVs were recovered (6551 out of 6796 SNVs). b,c, Bar plot showing the distribution of (b) indel and (c) HDR rates calculated for each experimental pool. HDR rates are calculated based on the percentage of reads for SNVs with fixed PAM modification in each experimental pool. d, Pearson correlation between the read counts of all SNVs recovered after HDR at a frequency of 1 in 105 reads in both replicates across the experimental pools.
