Extended Data Fig. 3: SGE experiments reveal temporal depletion of Cas9-induced indels.
From: Saturation genome editing-based clinical classification of BRCA2 variants
Histogram showing the distribution of indels identified in each experimental pool, along with their corresponding function scores. In this analysis, we considered indels that met specific criteria: they had to align with the reference genome, displaying a single insertion or deletion within 50 base pairs of the anticipated Cas9 cleavage site defined by the sgRNAs used in each experimental pool. These indels were only counted if their frequency was at least 1 in 106 reads in both replicates, and in-frame indels were marked when their size was divisible by 3. Function scores were determined by comparing the frequency of indels at day 14 relative to day 3. We observed depletion of frameshifting indels in the pool, while a few in-frame indels were occasionally tolerated.
