Extended Data Fig. 16: Lineage potential of human glial progenitors.

a, Schematic of the slice transplantation assay for glial progenitors. Created in BioRender. Wang, L. (2024) BioRender.com/t85o210. b–d, Immunostaining of progenies after progenitor transplantation to acute cortical slices on days in vitro 8. e, Quantification of progeny types after progenitor transplantation to acute cortical slices (n = 5, 5, 5, 5 cultures), including IPC-EN (EOMES⁺), EN (NeuN⁺), Tri-IPC (OLIG2⁺EGFR⁺), astrocyte (SPARCL1+), OPC or oligodendrocyte (OLIG2⁺EGFR⁻), and IPC-IN or IN (DLX5⁺). f, Schematic of the in vivo transplantation assay for glial progenitors. Created in BioRender. Wang, L. (2024) BioRender.com/f96w125. g, Immunostaining of progenies after progenitor in vivo transplantation into mouse cortex (n = 2 injections). White arrows indicate HNA⁺GABA⁺ inhibitory neurons. HNA, human nuclear antigen; L2-3, layer 2-3; L6, layer 6; WM, white matter; V-SVZ, ventricular-subventricular zone; OB, olfactory bulb. h, Immunostaining of progenies after progenitor in vivo transplantation into mouse cortex (n = 2 injections). White arrows indicate HNA⁺SOX10⁺ OPCs or oligodendrocytes. Yellow arrows indicate HNA⁺GFAP⁺ astrocytes. HNA, human nuclear antigen; L2-3, layer 2-3; L6, layer 6; WM, white matter; V-SVZ, ventricular-subventricular zone; OB, olfactory bulb.