Fig. 4: Multipotent progenitors during transition from neurogenesis to gliogenesis.
From: Molecular and cellular dynamics of the developing human neocortex
a, The expression patterns of surface proteins used for progenitor isolation. b, Schematic of the sorting strategy for isolation of progenitor subtypes (left). Right, phase-contrast images of progenitor subtypes after 5 days in culture. iSVZ, inner SVZ; oSVZ, outer SVZ; CP and SP, cortical plate and subplate. The diagram was created using BioRender. Scale bar, 50 μm. c, The proportion of individual cell types across progenitor subtypes and differentiation stages during progenitor differentiation in vitro. d, Clonal analysis demonstrating multipotency of individual progenitor cells. n = 26, 29 and 22 clones across three independent experiments. Scale bar, 100 μm. e, Immunostaining of descendants of Tri-IPCs 12 weeks after transplantation into mouse cortex, demonstrating the presence of astrocytes (GFAP+), OPCs or oligodendrocytes (SOX10+), and INs (GABA+). n = 2 injections. HNA, human nuclear antigen. The diagram was created using BioRender. Scale bar, 10 μm. f, SingleCellNet-predicted identities of INs and astrocytes derived from Tri-IPCs. g, Graphical summary of cell lineage relationships in late second-trimester human neocortex. The diagram was created using BioRender. h, UMAP plots of malignant GBM cells colour coded by SingleCellNet-predicted cell types. i, UMAP plots of malignant GBM cells colour coded by their main cellular states. j, The proportion of predicted cell types across different cellular states in malignant GBM cells. The legend is shown in h.
