Extended Data Fig. 5: GZMK cleavage of additional complement components.
From: GZMK-expressing CD8+ T cells promote recurrent airway inflammatory diseases
Representative Coomassie Blue-stained gels showing GZMK cleavage of serum-purified C2 (a), C4 (d) and C5 (g), as confirmed by Edman sequencing (b, e and h) and immunoblots (c, f and i). The incubation times are indicated. j, Hemolytic assays to measure the activity of serum-purified C5b6 with or without GZMK cleavage (See Methods for more details). Lysis of chicken erythrocytes were induced by the addition of C5b6 and an excess amount of serum-purified C7, C8 and C9. AU, arbitrary unit. Error bars indicate means± S.D. Each symbol denotes a technical replicate (n = 3), and lines denote means. Data are representative of at least two independent experiments. Source images for gels and blots are presented in Supplementary Fig. 1.
