Extended Data Fig. 6: Cryo-EM analyses of GGCX–BGP–MK-4.
From: Structure and mechanism of vitamin-K-dependent γ-glutamyl carboxylase
a, Summary of cryo-EM data processing procedures of the GGCX–BGP–MK-4 complex. b, Fourier shell correlation (FSC) curves between two half maps of the GGCX–BGP–MK-4 complex. c, Local resolution of cryo-EM map of the GGCX–BGP–MK-4 complex. Maps are colored according to local resolution, estimated using cryoSPARC. d, Cryo-EM map of GGCX–BGP–MK-4 (unsharpened) at different contour levels. The strong cholesterol (yellow) is visible at a high contour level, while the unassigned lipid/detergent densities (cyan) are invisible. e, Comparison of the Gla-binding site in GGCX–BGP and GGCX–BGP–MK-4 cryo-EM maps. GGCX, the propeptide and Gla domain of BGP were colored in grey, brown, and orange, respectively. The Gla peptide observed in GGCX–BGP structure but not in GGCX–BGP–MK-4 structure was indicated by a red arrow. f, Cryo-EM maps of the major structural elements of GGCX and BGP, as well as the cholesterol and PC in GGCX–BGP–MK-4 complex.
