Extended Data Fig. 8: Sensitivity of hMPC C54AMPC2 to inhibitors and comparison of inhibitor binding pockets between human MPC1/2 and yeast MPC1/3.
From: Structure of mitochondrial pyruvate carrier and its inhibition mechanism
a, The sensitivity of the yeast growth to inhibitor UK5099 for yeasts expressing human MPC WT and C54AMPC2 (mean ± SD; n = 3 independent experiments). b, The sensitivity of the yeast growth to inhibitor AKOS for yeasts expressing human WT and C54AMPC2 (mean ± SD; n = 3 independent experiments). c, The residues that differ in the UK5099 binding pocket of hMPC and yMPC. The predicted yMPC (MPC1/MPC3) structure, modeled based on hMPC structure, was superimposed onto the hMPC structure. The differing residues in the inhibitor-binding pocket are shown as sticks in green (hMPC1), cyan (hMPC2), and purple (yMPC). d, The sensitivity of the yeast growth to inhibitor UK5099 for yeasts expressing WT MPC and mutants MPC (mean ± SD; n = 3 independent experiments).
