Extended Data Fig. 2: Purification of MPC complex and structural comparison of MPC and SemiSWEET.
From: Structure of mitochondrial pyruvate carrier and its inhibition mechanism
a, Size-exclusion chromatography profile of the heterodimer of MPC1 and MPC2 and SDS-PAGE analysis. The experiments were repeated independently six times with similar results. For gel source data, see Supplementary Fig. 1a. b, Size-exclusion chromatography profile of the complex of MPC-NbS1755-Legobody in detergent and SDS-PAGE analysis. The experiments were repeated independently six times with similar results. For gel source data, see Supplementary Fig. 1b. c, Cryo-EM density map of the complex of MPCUK5099-NbS1755-Legobody. MPC1, MPC2, NbS1755, Legobody, and nanodisc are colored in green, cyan, orang, gray, and purple, respectively. d, Structure comparison of hMPC and SemiSWEET.
