Extended Data Fig. 10: Combinations of CIM-834 with GS-441524 or nirmatrelvir results in additive antiviral activity in vitro.
From: A coronavirus assembly inhibitor that targets the viral membrane protein
An antiviral assay was performed by infecting A549ACE2+TMPRSS2-mCherry with a SARS-CoV-2-mNeonGreen reporter virus as described previously41. Left, dose-response matrix for a, CIM-834 and GS-441524 or b, CIM-834 and nirmatrelvir representing average %inhibition of virus replication. Right, heat map of the delta scores (%) for the same combinations and ZIP analysis where δ = 0, δ > 0, and δ < 0 correspond to zero interaction, synergy, and antagonism, respectively. The overall zero interaction potency (ZIP) score represents the response beyond expectation (in %). In the range −10 < ZIP < 10, the compounds are likely to act in an additive manner, Score ≥10 indicate synergism. The results shown represent the means of two independent experiments for each combination. Data were then analysed with the SynergyFinder webtool80 based on zero interaction potency (ZIP) model.
