Extended Data Fig. 8: Reptin and Pontin knockdown in flies lengthens the circadian period or disrupts rhythmicity.
From: The P-loop NTPase RUVBL2 is a conserved clock component across eukaryotes
a, Quantification of percent rhythmicity for Pontin and Reptin RNAi knockdown flies. Black bar represents the % of rhythmic individuals and the grey bar represents the % of arrhythmic individuals in each genotype. b, Period of Drosophila lines with Pontin or Reptin RNAi expressed in all clock cells (crossed with tim-GAL4 driver flies), in Pigment Dispersing Factor (PDF) expressing brain neurons (crossed with pdf-GAL4 driver flies), or not expressed (crossed with w1118 flies). w1118 flies as control. n = 8, 16, 31, 14 for w1118, UAS-siRept#1;w1118, UAS-siRept#1;tim-GAL4 and UAS-siRept#1;pdf-GAL4. n = 11, 20, 31, 25 for w1118, UAS-siRept#2;w1118, UAS-siRept#2;tim-GAL4 and UAS-siRept#2;pdf-GAL4. n = 8, 11, 32, 9 for w1118, UAS-siPont#1;w1118, UAS-siPont#1;tim-GAL4 and UAS-siPont#1;pdf-GAL4. n = 11, 15, 15, 14 for w1118, UAS-siPont#2;w1118, UAS-siPont#2;tim-GAL4 and UAS-siPont#2;pdf-GAL4. In certain genotypes, the free-running period was calculated separately for the initial 4 days of DD (1-4 DD) and the last three days (5–7 DD) to detect arrhythmicity that was prominent after day four. The Drosophila melanogaster locomotor activity was analyzed by ClockLab Analysis. The data show mean ± s.e.m.
