Extended Data Fig. 2: A genetic screen of RUVBL2 in U2OS cells.
From: The P-loop NTPase RUVBL2 is a conserved clock component across eukaryotes
a, Normalized luminescence expression in Per2-dLuc U2OS cells with or without stable Cas9 expression, n = 3 biologically independent cells. b, The effects of siPER2 and siACTB on Per2-dLuc U2OS cells. Data were reanalyzed from Zhang, E.E. et al.19 Cell. c, Compilation of mutation information for represented RUVBL2 and PER2 mutants in Fig. 1f. d, Compilation of in-frame (left) and frame-shift (right) mutation information for RUVBL2 mutants in secondary validation. e, The procedure of validation across entire screen. All period data parameters were analyzed from LumiCycle Analysis. All graphs show mean ± s.e.m.
