Extended Data Fig. 4: ATP hydrolysis activity of RUVBL and KaiC proteins governs the clock period.
From: The P-loop NTPase RUVBL2 is a conserved clock component across eukaryotes
a, SDS-PAGE image of human RUVBL1/2 and cyanobacterial KaiC proteins. For gel source data, see Supplementary Fig. 1b. b, Alignment of the secondary structures of human RUVBL1/2 and cyanobacterial KaiC proteins. c, Eukaryotic RUVBL1/2 (in the HslU-ClpAB/CTD-LonAB-RuvB clade) and cyanobacterial KaiC (RecA homolog) genes belong to the P-loop NTPase superfamily (Figure is adapted from Erzberger et al Ann Rev Biophys Biomol Struct 2006). d, The structures of cordycepin triphosphate and adenosine triphosphate. e, Dose effects of cordycepin on the luminescence rhythms in Per2-dLuc U2OS cells in the presence of pentostatin, which prevents the rapid degradation of cordycepin in the human cells. n = 3 biologically independent cells. One-way ANOVA differential analysis with Šídák’s multiple comparisons test. All period data parameters were analyzed from Lumicycle Analysis. All bar graphs show mean ± s.e.m. Credit: c adapted with permission from ref. 35, Annual Reviews.
