Extended Data Fig. 7: Two-photon imaging quantification in PT and IT cells.
From: Psilocybin’s lasting action requires pyramidal cell types and 5-HT2A receptors
a, Schematic illustrating how fluorescent transients are processed and analysed to derive event rate, amplitude, and frequency. b, Fractional change in frequency detected in dendritic branches of PT neurons (top row) and dendritic branches of IT neurons (bottom row) after psilocybin (PT, yellow; IT, purple) or saline (PT, grey; IT, light purple). c, Similar to (b) for amplitude. d, e, Similar to (b, c) for dendritic spines. PT: n = 140 branches from 4 mice (Saline), n = 149 branches from 4 mice (Psilocybin). IT: n = 90 branches from 3 mice (Saline), n = 95 branches from 3 mice (Psilocybin). ** p < 0.01. ***, p < 0.001, post hoc with Bonferroni correction for multiple comparisons. Detailed sample size n values are provided in Methods. Statistical analyses are provided in Supplementary Table 1.
