Extended Data Fig. 8: Spatial proximity between basal-like tumour cells and myCAFs.

a, Co-immunostaining of PanCK (green), S100A2 (red), and DAPI (blue) in matched liver and lung metastases of three different KPCY mice. White lines denote the tumour bed, and yellow lines denote the juxtalesional areas. b, Mean intensity of α-SMA across individual cells in juxtalesional ROIs between liver (LiM) and lung (LuM) metastases. For mouse 1, n = 86,763 cells (LiM) and n = 4,125 cells (LuM). For mouse 2, n = 21,310 cells (LiM) and n = 5,101 cells (LuM). For mouse 3, n = 194,729 cells (LiM) and n = 11,861 cells (LuM). Each dot with connected lines represents data from the same mouse. Student’s paired t-test. ****P < 0.0001. c, A representative image of E-cadherin (white), α-SMA (green), S100A2 (red), and GATA6 (blue) in a liver metastasis containing both classical-like and basal-like tumour cells. White arrows with numbers denote the enlarged areas. d, Phase contrast, H&E staining, and co-immunostaining for S100A2 (red), α-SMA (green), and DAPI (blue) in classical-like and basal-like PDOs cultured with human CAFs. Full PDO IDs are as follows: 185; PANFR0185_T2, 332; PANFR0332_T1, 172; PANFR0172_T4, and 440; PANFR0440_T1. e, Quantification of the percentage of organoids displaying direct attachments of α-SMA+ CAFs in each PDO line. Each dot represents an individual CAF line. Data are presented as mean ± SD (n = 3, independent experiments). Student’s unpaired t-test. *P < 0.05 (P = 0.014 in 185 vs. 172, P = 0.01 in 185 vs. 440), **P < 0.01 (P = 0.006 in 332 vs. 172, P = 0.005 in 332 vs. 440).