Extended Data Fig. 4: FISH validation of MYC (8q24.2).
From: Spatial mapping of transcriptomic plasticity in metastatic pancreatic cancer
a,e, Spatial visualization of subclones from Fig. 2, along with fluorescence images for MYC (red) and control (Ctrl, green) probes targeting the centromere of chromosome 8 in Pt-10 (a) and Pt-1 (e). Dotted boxes and numbers indicate the enlarged areas. b,f, MYC CNV scores from the inferCNV analysis are shown in b (Pt-10) and f (Pt-1). Each dot represents each subclone. Data are presented as mean ± standard deviation (SD). For Pt-10 (b), clone A (n = 2), clone B (n = 3), clone C (n = 5) and clone D (n = 1). For Pt-1 (f), clone A (n = 2), clone B (n = 3) and clone C (n = 5). c,g, Per-cell copy number (CN) ratio for MYC in Pt-10 (c) and Pt-1 (g). d,h, Percentages of cells with MYC amplification (CN ratio over 1) in Pt-10 (d) and Pt-1 (h).
