Extended Data Fig. 4: In vivo detection of TRAIL+ astrocytes.
From: Glioblastoma-instructed astrocytes suppress tumour-specific T cell immunity
(a,b) Flow cytometry gating strategy for analysis of TdTomato+ TRAIL+ cells in Aldh1l1Cre-ERT2/TdTomato reporter mice (a) and analysis of TRAIL expression in TdTomato+/− fractions (b). Paired two-tailed t-test. (c) Immunofluorescence analysis of the tumour border in GL261 tumour-bearing Aldh1l1eGFP reporter mice at day 15 (left). The fluorescence signals of DAPI, TRAIL, GFP astrocyte reporter are shown along with a quantification of TRAIL+ cells within GFP+ or GFP- fractions (right). (d) Immunofluorescence analysis and quantification of TRAIL+ astrocytes in naïve or sham-injured mice after 15 days. Data shown as mean ± SEM. Unpaired two-tailed t-test used for statistical analysis. n indicates biologically independent samples.
