Extended Data Fig. 7: Analysis of pSTAT3+ TRAIL+ astrocytes.
From: Glioblastoma-instructed astrocytes suppress tumour-specific T cell immunity
(a) TNFSF10 expression in human astrocytes stimulated overnight with tumour-conditioned or control medium from three patient-derived GBM lines (BT145, BT482, BT1018). (b) Analysis of calcium signalling in astrocytes by use of Fluo-4-AM calcium indicator in primary mouse astrocytes stimulated in vitro with the indicated conditions. (c) Pearson correlation of STAT3 signalling programs (M5897) and TNFSF10 expression in Ivy Glioblastoma Atlas bulk RNA-seq data split by anatomical structure. (d) pSTAT3 measurement by flow cytometry in TRAIL+ or TRAIL- astrocytes from Aldh1l1Cre-ERT2/TdTomato reporter mice implanted with MK007. (e) TNFSF10 expression and STAT3 (Y705) phosphorylation in GBM samples from CPTAC-3 dataset. Only patients that ultimately succumbed to GBM and not any other morbidity were analysed. Boxplot with median, interquartile range (IQR) ± 1.5IQR, and minimal/maximal values is shown. (f) STAT3 binding sites within 2 kb upstream of the transcription start site (TSS) in the human TNFSF10 predicted by MULAN. (g) pSTAT3 (Y705) in GL261 TCM-treated murine astrocytes. Data shown as mean ± SEM. Unpaired two-tailed t-test used. n indicates biologically independent samples.
