Extended Data Fig. 3: scRNA-seq analysis of astrocytes in the GL261 model.
From: Glioblastoma-instructed astrocytes suppress tumour-specific T cell immunity
(a) In vivo bioluminescence signal used to quantify luciferase-expressing GL261 tumour size at 7 and 14 days after implantation. Net photons per second (P/s) after background correction are shown. Boxplot shows interquartile range with mean, minimal and maximal values. (b-d) Violin plots of quality control metrics for GL261 Aldh1l1cre-ERT2/TdTomato reporter cell scRNA-seq showing number of detected genes (b), number of counts (c), and percent of mitochondrial reads (d). (e) Elbow plot of principal component analysis. (f) Heatmap of cluster markers. (g) Feature plots showing expression of canonical astrocyte markers Gfap, Aldoc, Slc1a2, Slc1a3, Aldh1l1, S100b, Gja1 and Vim in mouse scRNA-seq. (h,i) UMAP plot split by condition (h) and cluster proportions by sample (i). (j) MiloR differential abundance results showing the fold change (FC) of neighborhood abundance in GL261 vs. Control samples. Neighborhoods are grouped by cluster. (k) GSEA pathway analysis of cluster 1 cells in GL261 scRNA-seq data. NES, normalized enrichment score. n indicates biologically independent samples.
