Extended Data Fig. 5: The quality assessments of cryo-EM maps.
From: Substrate recognition and cleavage mechanism of the monkeypox virus core protease
Cryo-EM densities for the indicated regions are shown: a, residues surrounding the active site of MPXV CorePro in the apo-form (Threshold: 0.4), the aloxistatin binding region (Threshold: 0.4), and the I-G18 binding region (Threshold: 0.6); b, The dimer interface of MPXV CorePro in the apo-form (Threshold: 0.7); c, Residues from Asn321 to Cys328 of MPXV CorePro in the apo-form and in complex with aloxistatin (Threshold: 0.4); d, The regions that can only be traced in CorePro-I-G18 complex (Threshold: 0.45). The structures of MPXV CorePro in the apo-form, in complex with aloxistatin and in complex with I-G18 are in gray, dark slate blue, and forest green, respectively. The cryo-EM densities are shown as grey with 40% transparency. The residues showing densities are labelled nearby.
