Extended Data Fig. 10: Comparison of EPI-Clone and mitochondrial lineage tracing by scTAMito-seq.
From: Clonal tracing with somatic epimutations reveals dynamics of blood ageing
a. Static CpG UMAP computed on all cells from the patient, highlighting cell types identified using surface antigen expression levels. b. Average coverage in reads per cell for the mitochondrial variants previously described for donor X.246. c. Scatter plot comparing average heteroplasmies for these mutations, as determined by mt-scATAC-seq (reference46) or scTAMito-seq (this study). d. Scatter plot depicting, for all mitochondrial variants, the average heteroplasmy and the statistical association with EPI-Clone. Specifically, a linear model was trained on EPI-Clone clusters to predict heteroplasmy at the single cell level, and the p value from an F-test is shown. e. Heatmap relating the single-cell heteroplasmies of mitochondrial variants to EPI-Clones, for T cells only. The columns correspond to different T cells and the rows comprise mitochondrial mutations measured by scTAMito-seq.
