Extended Data Fig. 10: Stable LHX2 and EBF1 condensates with >48-h-old protein localize to the active OR allele in vivo.
From: Solid phase transitions as a solution to the genome folding paradox
LHX2 and EBF1 protein were stained with JF646 HaloTag Ligand and then incubated with 7BRO for 48 h to label all old protein with far-red fluorescence. TF hubs adjacent to Olfr17 DNA in Olfr17-expressing cells contain old ( > 48 h) protein. Line scans are marked on figures with dotted white lines and arbitrary intensity units are plotted over distance. Grey vertical lines indicate local maxima in signal for each channel. All neurons are 6-7 days old. (n = 5 cells each from 2 independent experiments, independent experiments show similar results). Scale bars are 5 μm.
