Extended Data Fig. 1: The proteins of the OR Hub co-bind to the composite motif.
From: Solid phase transitions as a solution to the genome folding paradox
a, Coomassie stained SDS-PAGE gel of recombinant truncated and FL LHX2, EBF1, and LDB1 proteins. This experiment was repeated independently at least three times with similar results. b, Schematic of the recombinant truncated and FL proteins purified. Tag schematics created with BioRender.com. c, Electromobility shift assay (EMSA) of reactions containing truncated LHX2 and EBF1 with LHX2 motif, EBF1 motif, and composite motif DNA. d, EMSA binding curves and Kd values from reactions containing truncated Sumo-LHX2 (top) and truncated EBF1 (bottom) with three different composite motifs from Halki, Sfaktiria, and Psara enhancers. Data are presented as mean values +/− SD, n = 3 independent experimental replicates. e, Example EMSA from reactions quantified in f. f, Binding curves and Kd values from reactions containing truncated composite motif DNA with LHX2 alone and in complex with EBF1 (top) and with EBF1 alone and with LHX2 (bottom). Data are presented as mean values +/− SD, n = 3 independent experimental replicates. g, EMSA of reaction containing truncated Sumo-LHX2, EBF1, and Sumo-LDB1-LID domain with composite motif DNA. This experiment was repeated three times with similar results. h, EMSA of reaction containing truncated Sumo-LHX2, EBF1 and FL LDB1 with composite motif. This experiment was repeated three times with similar results.
