Extended Data Fig. 4: LHX2 and EBF1 condensates do not exhibit liquid properties in vivo.
From: Solid phase transitions as a solution to the genome folding paradox
a,b, FRAP on endogenous LHX2 (a) and EBF1 (b) condensates in in Olfr17-expressing cells. Recovery is plotted over 10 min and represents 6 cells. Normalized intensity from an ROI within a bleached region (magenta) and within a control ROI from the same nucleus (grey) is plotted over 10 min and from 6 cells. Mean is plotted and error bars represent standard deviation. c–e, Representative images of changes to BRD4 (c), LHX2 (d), and EBF1 (e) condensates after treatment with 1,6-hexadiol. Pixel intensities were normalized for each nuclei and then 50,000 randomly selected pixels from 3 nuclei for each condition were combined to plot normalized pixel counts. Similar results have been obtained from 3 biologically independent experiments using different ligands. Scale bars are 5 μm.
