Fig. 1: Cryo-EM structure of human SIFI.
From: Molecular basis of SIFI activity in the integrated stress response
a, Cryo-EM maps of SIFI, highlighting its C-terminal partial map (endogenous SIFI complex; top left; Electron Microscopy Data Bank (EMDB): EMD-46686, contour level 0.12) and N-terminal partial map (immunoprecipitation (IP) of KCMF1; bottom left; EMDB: EMD-46688, contour level 0.07). (A) and (B) denote the two heterotrimers. b, Composite structure of human SIFI built from partial maps (Protein Data Bank (PDB): 9D9Z), including two subunits each of UBR4 (blue), KCMF1 (purple), calmodulin (green) and UBE2A (red). c, Surface representations of the SIFI complex viewed from the N- and C-terminal dimerization regions, as well as views from above and the side of ring’s plane. All components are colour coded as in a and b.
