Extended Data Fig. 10: CREM KO CAR70/IL-15 NK cells do not cause toxicity in mice in a patient-derived xenograft (PDX) mouse model of metastatic breast cancer.
From: CREM is a regulatory checkpoint of CAR and IL-15 signalling in NK cells
(a) Hematoxylin and eosin [H&E] stained tissues from mice treated with either CREM KO CAR70/IL-15 NK cells (in BCX.010 tumor-bearing (n = 5 mice) or non-tumor bearing (n = 3 mice) mice) or CREM WT CAR70/IL-15 NK cells in BCX.010 bearing mice (n = 3 mice). Mice were euthanized on day 14 post-treatment. Magnification: 10x. Scale bar: 100 μm; (b-d) Assessment of blood cell populations (b), kidney (c) and liver (d) function parameters in the blood of mice engrafted with BCX.010 tumors, at day 30 post-treatment with CREM WT CAR70/IL-15 NK cells (n = 4 mice) or CREM KO CAR70/IL-15 NK cells (n = 5 mice). WBC, white blood cells; HGB, hemoglobin; BUN, blood urea nitrogen; ALP, alkaline phosphatase; ALT, alanine transaminase; AST, aspartate aminotransferase; LDH, lactate dehydrogenase; ns, non-significant. Statistical comparisons were performed using Student’s t-test (b-d). Data are represented as mean ± SEM.
