Extended Data Fig. 4: CREM is induced in NK cells stimulated by certain cytokines and through CD16, NKp30, and NKp46.
From: CREM is a regulatory checkpoint of CAR and IL-15 signalling in NK cells
(a) CREM expression in NK cells stimulated with increasing concentrations of indicated cytokines as assessed by qPCR (n = 3 donors); (b) Whole cell lysates from NK cells that were either unstimulated or stimulated with plate-bound anti-CD16, anti-NKp30, or anti-NKp46 antibodies for 30 min were analyzed by western blot for pCD3ζ as well as total CD3ζ. β-actin was used as loading control for the pCD3ζ gel (n = 2 donors); (c) CREM expression in NK cells that were either unstimulated or stimulated with plate-bound anti-CD16 (αCD16), anti-NKp30 (αNKp30), or anti-NKp46 (αNKp46) antibodies for 24 h, assessed by qPCR (n = 9 donors). unstim: unstimulated; ns: non-significant. Statistical comparisons were performed using one-way ANOVA individually for each cytokine (a) and one-way ANOVA (Fisher’s LSD test, c). Data are represented as mean ± SEM.
