Fig. 2: Improving catalytic efficiency through low-throughput screening of FuncLib designs.
From: Complete computational design of high-efficiency Kemp elimination enzymes
a, Catalytic efficiencies of 12 FuncLib designs encoding 5–8 active-site mutations relative to Des27. Data represent mean ± s.d. of 2–5 biological replicates, except for Des27.2 and Des27.3 (n = 1). b, Michaelis–Menten analysis of Des27.7. Data are the mean of two technical repeats. c, The crystal structure of the ligand-unbound Des27.7 (grey, PDB entry 9HVB) verifies the accuracy of the designed active site (blue) with r.m.s.d. < 0.5 Å.
