Extended Data Fig. 1: Expression and stability of the initial design round.
From: Complete computational design of high-efficiency Kemp elimination enzymes
a. 66 designs were solubly expressed. High-functioning designs were expressed independently 2–5 times; otherwise, protein expression was performed once. For gel source data, see Supplementary Data 1. b. Temperature melts of representative cooperatively folded (left) and unfolded (right) designs. All temperature melts are performed in technical duplicates.
