Extended Data Fig. 7: Pde5-cGMP-mediated DC migration in inflammation and homeostasis.
From: Rescuing dendritic cell interstitial motility sustains antitumour immunity
a, Representative flow cytometric plots and quantifications of FITC+ migratory and resident cDCs in tdLNs from Pde5fl/fl and Zbtb46crePde5fl/fl mice (Pde5fl/fl, n = 6; Zbtb46crePde5fl/fl, n = 7). Mice were painted with FITC-LPS mixture and tdLNs were analysed 48 h post painting to assess FITC uptake. b, Quantifications of cDC percentages, CCR7 expression, or surface CD80/CD86 expression from Pde5+/+ and Pde5−/− BMDCs differentiated with FLT3L for 9 days (n = 3 biological replicates per group). c,d, Quantifications of migratory cDCs (c), and migratory XCR1+ and SIRPα+ cDCs (d) in mediastinal LNs from Pde5fl/fl and Zbtb46crePde5fl/fl mice at homeostasis (n = 6 per group). e, Quantification of total cDCs in lung tissue from Pde5fl/fl and Zbtb46crePde5fl/fl mice (n = 6 per group). Cells were gated on live CD45+CD3−B220−Gr1−F4/80−. Data are representative of at least two to three independent experiments and present as mean ± s.e.m. Statistical analysis was performed using two-tailed Student’s unpaired t-test.
