Extended Data Fig. 6: Evaluation of NINJ1 K45Q mutant’s effect on plasma membrane rupture under mechanical strain.
From: NINJ1 regulates plasma membrane fragility under mechanical strain
a, The relative mRNA levels of NINJ1 WT and NINJ1 K45Q in overexpressed in HeLa cells. 3 replicates were done for each group. n = 3 trials per group. One-way ANOVA followed by Bonferroni corrections. ** p <0.01 versus vector. b, c, LDH release and the percentage of DRAQ7+ HeLa cells overexpressing vector control, NINJ1 WT and NINJ1 K45Q, after mechanical stimulation at various strain levels. n = 3 ~ 6 trials each group. 2-way ANOVA followed by Bonferroni corrections. * p < 0.05, ** p <0.01 versus vector at the same strain level. All data are presented as mean ± s.e.m.
