Extended Data Fig. 7: The pyroptotic THP-1 cells maintain the ballooned morphology in the absence of mechanical stimulation.
From: NINJ1 regulates plasma membrane fragility under mechanical strain
a, Western blot of endogenous NINJ1 of THP-1 cells on a native BN-PAGE gel with or without incubation with 5 µg ml−1 Nigericin for 90 min. b, THP-1 cells were incubated with 5 µg ml−1 Nigericin and stained for plasma membrane (CellBrite, red), nuclei of cells with compromised membrane (DRAQ7, yellow) and all nuclei (Hoechst, blue) 48 h later. Cells with permeabilized plasma membrane showed DRAQ7/Hoechst double staining, represented in white. Scale bar, 50 µm. These experiments have been repeated at least 3 times independently with similar results.
