Fig. 3: NINJ1 levels on the plasma membrane are inversely correlated with rupture tension.
From: NINJ1 regulates plasma membrane fragility under mechanical strain
a, Schematic of the micropipette aspiration assay. HeLa cells expressing NINJ1–mCherry fusion were treated with 2 mM NEM to generate GPMVs, which were aspirated through a micropipette to determine the lysis tension. Components of the diagram were created by Z. Zhuoyi Xu and using BioRender. b, Representative images of GPMVs with high or low NINJ1–mCherry fluorescence levels collected during the aspiration protocol. GPI–eGFP was used as a membrane marker. Scale bar, 10 µm. c, Traces showing the volume changes in NINJ1high and NINJ1low GPMVs during the pressure ramping. d, Lysis tension of the GPMVs as a function of membrane NINJ1–mCherry fluorescence. GPMVs were generated from HeLa cells overexpressing WT NINJ1–mCherry (n = 30 vesicles), K45Q mutant NINJ1-mCherry (n = 8 vesicles) or GPI–eGFP alone (n = 10 vesicles) across three independent experiments. Unless otherwise indicated, data are mean ± s.e.m. Error bars that were too small, or out of the logarithmic scale, are not displayed.
