Extended Data Fig. 4: Assessment of ectopic and endogenous NINJ1 expression in various cell lines.
From: NINJ1 regulates plasma membrane fragility under mechanical strain
a, Relative NINJ1 mRNA level of vector- and CMV-NINJ1-IRES-mCherry-transfected HEK-293T cells 24 h post-transfection. n = 3 for each group. Two-sided unpaired Student’s t-test, ** p < 0.01 vs Vector. b, Representative image of HEK cells overexpressing NINJ1-mCherry fusion under the CMV promoter 24 h post-transfection. Fluorescent puncta were visible (arrowheads) and plasma membrane ballooning were evident in some cells (arrows). NINJ1 was visualized with direct mCherry fluorescence. Nuclei were live stained with Hoechst. Scale bar, 25 µm. c, Relative NINJ1 mRNA levels of HEK-293T parental cells and the cells stably expressing NINJ1 driven by TRE3G promoter. mRNAs were measured 24 h after induction by Doxycycline (Dox) of various doses. n = 4 trials per group. One-way ANOVA followed by Bonferroni corrections. ** p <0.01 versus Parental. d, Western blot of endogenous NINJ1 from HeLa and ZOS cells. 3 replicates were done for each cell line. e, Relative NINJ1 mRNA level of HeLa and ZOS cells. 3 trials were conducted for each group. Two-sided unpaired Student’s t-test, ** p <0.01 versus HeLa. Unless otherwise noted, all data are presented as mean ± s.e.m.
