Extended Data Fig. 2: Cryo-EM data processing workflow for ROOLEfa.

a, Maximum-likelihood classification of cryo-EM data using cryoSPARC. b, Example of a micrograph with ROOLEfa particles. 6,966 micrographs were obtained and analysed. c, Fourier shell correlation curve as a function of resolution for the final map shown in panels a and e. d, Euler angle distribution of particles contributing to the final reconstruction. e, Cryo-EM maps coloured according to local resolution.